Map of panama single color is a fully layered, editable vector map file. Contour map of the middle east in vector format, illustrator and svg. The seeds were germinated in plastic pots containing garden soil and the plants were grown in natural conditions. Download thousands of royalty free vector maps, world maps and map bundles in adobe illustrator, powerpoint, eps, pdf, png and jpg formats. The map, notes, and annotations on this page and in the sequence map file are ed material. The exact position of genetic elements is shown on the map termination codons included. Note the specific antibiotic to be used with this vector. This vector is designed for cloning and generation of exoiii deletions. The puc18 and puc19 plasmids enable successful cloning of large dna fragments larger than those cloned with a m mp18 rf phage vector. These changes together result in a temperaturedependent copy number of. Map of the glutathione stransferase fusion vectors showing the reading frames and main features. Atg start codon present on the pet translation vectors. Naturallyoccurring plasmids are viruses of bacteria.
Terms and conditions 20042018 harvard medical school plasmid was created and is maintained by the dfhcc dna resource core at harvard medical schooldfhcc dna resource. Description the pgl4 luciferase reporter vectorsae are the next generation of reporter gene vectors optimized for expression in mammalian cells. It contains the pmb1 origin of replication from pbr322, but it lacks the rop gene and carries a point mutation in the rnaii transcript g 2975 in pbr322 to a 8 in puc19. New vectors for high level expression of recombinant proteins in bacteria david j. This page is informational only this vector is not available from addgene please contact the manufacturer for further details. Highlights purified by chromatography using proprietary patented technology more than. Vector database is a digital collection of vector backbones assembled from publications and commercially available sources.
New vectors for high level expression of recombinant proteins. To the extent possible under law, uploaders on this site have waived all to their vector images. A vector file ai, pdf has flexible tools for displaying elements of maps you can discrete or arbitrary scaling map performing group operation with selected features. The vector also carries the pbr322derived cole1 replicon, laci gene and ampicillin resistance gene. This listings of restriction sites for the pgl2 luciferase reporter vectors are provided in sections 7. The vector contains two multiple cloning sites mcs, each of which is preceded by a t7 promoterlac operator and a ribosome binding site rbs. Confirm sequencing primer sequences match vector before sequencing.
These cloning vectors contain a multiple cloning site at the lacz region that enables recombinant plamids to be verified via bluewhite colony screening using agar plates containing iptg and xgal. The expression efficiency of recombinant human tumor necrosis factor. The linearized ptg19t vector with 3dt overhangs prevent vector recircularization, therefore resulting in high percentage of recombinant clones and low background. Thermo scientific puc18 vector is a small, high copy number, e. Gleave ap 1992 a versatile binary vector system with a tdna organisational structure conducive to efficient integration of cloned dna into the plant genome. Description the ptg19t vector is designed for rapid and efficient cloning of pcr products with 3da overhangs. It contains identical multiple cloning site mcs as puc19 vector except that it is arranged in opposite orientation. Note that the sequence is numbered by the pbr322 convention, so the t7 expression region is. Expression of target dna is enabled by the presence of a. For cloning vector part7 and binary vector part27 see. To see this sequence with restriction sites, features.
Note that the sequence is numbered by the pbr322 convention, so the t7 expression region is reversed on the circular map. The artificial plasmid puc18 has been genetically engineered to include 1 a gene for antibiotic resistance to ampicillin amp r, and 2 a gene and its promoter for the enzyme betagalactosidase lacz. Biodefense and emerging infections research resources repository bei resources is the leading source for reagents, tools and information for studying niaids category a, b, and c priority pathogens and emerging infectious disease agents. The coordinates refer to the position of the first nucleotide in each. Even though stop codons in all three frames are not depicted in this map, all thirteen vectors have stop codons in all three frames downstream from the multiple cloning site. You are free to edit, distribute and use the images for unlimited commercial purposes without asking permission. Each vector has a large multiple cloning site in three reading frames to simplify cloning in frame with the nterminal secretion signal. The molecule is a small doublestranded circle, 2686 base pairs in length, and has a high copy number. The cloningexpression region of the coding strand transcribed by t7 rna polymerase is shown below. A repository of over 200,000 plasmids including protein structure initiative protein expression plasmids and vectors, over 75,000 human plasmids, and whole genome collections from many organisms. Free vector world maps available in adobe illustrator, eps, pdf, png and jpg formats to download.
Vector map of panama single color free vector maps. A plasmid is a circular dsdna molecule a few hundred or thousand base pairs in circumference. Click on the button download pdfsvg and look for the downloaded file in your downloads folder. The pcr amplified products were prepared with a overhang and cloned into the ptz57rt vectors adopting manufacturers protocols mbi fermentas, hanover, md, usa.
Dixon department of biological chemistry, university of michigan medical school, ann arbor, michigan 481090606 received october 4,1991 a system has been developed for synthesis and rapid. Plasmid puc57, 2710 bp in length, is a derivative of puc19. See vector map perform a cotransfection of the recombinant plasmid and bd baculogold linearized baculovirus dna cat. Thermo scientific puc19 vector is a small, high copy number, e. This is a free resource for the scientific community that is compiled by addgene. The ptz57rt vector was obtained from mbi fermentas. It contains identical multiple cloning site mcs as puc18 vector except that it is arranged in opposite orientation. The puc18 puc19 sequence is stored as a pdf file on the genaxxon webpage. The cut sites for some restriction enzyme are indicated on the plasmid.
395 65 342 1442 834 602 1125 523 1414 767 563 541 1397 492 895 845 345 537 502 1193 203 823 382 1176 388 1305 1206 1506 153 1119 428 599 1130 1145 1075 691 606 288 548